Xu, Qin, Noriyuki Ohara, Wei Chen, Jin Liu, Hiroko Sasaki, Akira Morikawa, Régine Sitruk-Ware, Elof D.B. Johansson, and Takeshi Maruo. 2006. “Progesterone receptor modulator CDB-2914 down-regulates vascular endothelial growth factor, adrenomedullin and their receptors and modulates progesterone receptor content in cultured human uterine leiomyoma cells,” Human Reproduction 21(9): 2408–2416.

Background
This study was conducted to evaluate the effects of graded concentrations (10^–8, 10^–7 and 10^–6 M) of progesterone receptor (PR) modulator CDB-2914 on the protein contents of PR, of vascular endothelial growth factor (VEGF), adrenomedullin (ADM), and their receptors in cultured human uterine leiomyoma and matching myometrial cells.

Methods
PR-A, PR-B, VEGF-A, VEGF-B, VEGF receptor (VEGFR)-1, VEGFR-2, ADM, and ADM receptor (ADMR) contents were assessed by Western blot analysis.

Results
Treatment with 100 ng/ml progesterone increased VEGF-A, VEGF-B and ADM contents in cultured leiomyoma cells and normal myometrial cells. The concomitant treatment with 10^–6 M CDB-2914 significantly decreased the progesterone-induced VEGF-A, VEGF-B, and ADM contents in cultured leiomyoma cells but not in normal myometrial cells. CDB-2914 treatment alone decreased VEGFR-1, VEGFR-2, and ADMR contents in cultured leiomyoma cells but not in normal myometrial cells. CDB-2914 treatment increased PR-A and decreased PR-B contents in cultured leiomyoma cells in a dose-dependent manner compared with untreated cultures, whereas no significant changes in PR isoform contents were observed in normal myometrial cells.

Conclusions
These results suggest that CDB-2914 down-regulates VEGF, ADM, and their receptor contents and modulates PR isoform contents in cultured leiomyoma cells in a cell-type-specific manner.

Return to Female Hormone Therapy Publications/Resources page

Print this page

@ E-mail this page

This page updated 26 October 2006

---