Abstract
Enhanced chemiluminescence (ECL) for routine immunoblotting. An inexpensive alternative to commercially available kits (PDF)
Mruk,Dolores D.; Cheng,Chuen-yan
Spermatogenesis 1(2): 121-122
Publication date: 2011
Immunoblotting is an analytical technique used by many laboratories to study protein expression. It involves electrophoretic separation of proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ), immobilization of these proteins onto a membrane of either nitrocellulose or polyvinylidene difluoride, incubation of the membrane in a monoclonal or polyclonal antibody and detection by a standard method such as enhanced chemiluminescence (EC L). To achieve this, most laboratories opt to use commercially-available chemiluminescence kits which are acceptable but relatively expensive. In this technical report, we show that a self-prepared chemiluminescence reagent is superior to a commercially obtained kit in terms of sensitivity, duration of signal, ease-of-use and shelf-life butat a fraction of the cost of a kit.
Get Involved
- Make a contribution to the Population Council
- Sign up to receive e-mail announcements
Connect
